Buffers for protein extraction
– RIPA
– NETN
Phosphatase and Protease inhibitors
Pyrogallol Red Molybdate precipitation
Immunoprecipitation (english) (french)
Affinity pulldown (tandem purification via SFB tag)
laminin overlay
Preparation polyacrylamide gels
– Bistris buffer system
– Tris-Glycin buffer system
– Tris-Acetate buffer system
Gel transfer
Blue Native Gel electrophoresis
– Variant A: Schaegger protocol
– Variant B: Wittig protocol (english) (french)
Western Blot
general protocol (english) (french)
Home-made enhanced chemoluminescence solution
Akta liquid chromatography usage
Production of soluble protein from bacteria
Purification of (HIS)6-tagged proteins
Purification of GST-tagged proteins
Self-made blue sepharose column
Production of recombinant ApoAI
Desalting using Sepharose minicolumns (Pernefsky)
Protein quantification:
– BCA assay
Coomassie staining (colloidal)