{"id":213,"date":"2022-05-16T17:32:59","date_gmt":"2022-05-16T17:32:59","guid":{"rendered":"http:\/\/chaletloree.com\/?page_id=213"},"modified":"2025-09-17T15:46:37","modified_gmt":"2025-09-17T15:46:37","slug":"cell-culture","status":"publish","type":"page","link":"http:\/\/chaletloree.com\/index.php\/cell-culture\/","title":{"rendered":"cell culture"},"content":{"rendered":"<div class=\"et_pb_section et_pb_section_0  et_pb_css_mix_blend_mode et_section_regular\" >\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_row et_pb_row_0  et_pb_css_mix_blend_mode\">\n\t\t\t\t<div class=\"et_pb_column et_pb_column_4_4 et_pb_column_0  et_pb_css_mix_blend_mode et-last-child\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_pb_text et_pb_text_0  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><!-- divi:html --><\/p>\n<p><strong><u>General maintenance<\/u><\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1wtNsoCxMgXlYRhw-YLGsDm9TyG9Yb1ulBNkt53DYIH8\/edit?usp=sharing\" title=\"\">general rules for the tissue culture room<\/a>\u00a0(english) and techniques (<a href=\"https:\/\/docs.google.com\/document\/d\/1VkNM62PFubmXoDmSjcQKPgqvslCD8u1MVo63Sw-Y-Gk\/edit?usp=sharing\" title=\"\">french<\/a>)<\/li>\n<li>mycoplasma PCR\u00a0(<a href=\"https:\/\/docs.google.com\/document\/d\/1mU1S8GqqfqVm8IYyRCukrxPdkNaH1kr8PbwLOppJK48\/edit?usp=sharing\" title=\"\">english<\/a>) (<a href=\"https:\/\/docs.google.com\/document\/d\/1mdN5_Zt5l3_XyC39CdxKAFon49ksOIp-W2jkLS7URDY\/edit?usp=sharing\" title=\"\">french<\/a>)\u00a0<\/li>\n<\/ul>\n<p><strong><u>Transfection and infections<\/u><\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/docs.google.com\/spreadsheets\/d\/1WOusPtOvdWxYiGoVBHR8O6DwJJ7yf9qaAgIKeCUNJqw\/edit?usp=sharing\" title=\"\">Production of recombinant Lentiviruses (excel sheet with calculations)<\/a><\/li>\n<li>Production of recombinant Retroviruses<\/li>\n<li><a href=\"https:\/\/docs.google.com\/spreadsheets\/d\/1dwdyJlJWVK8Yy0tPlPwmF2-Zu4QbC3iz4Mjg1CWZiDE\/edit?usp=sharing\" title=\"\">Transfection with siRNA and plasmid together<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1U_MKw8HfHTwYCS5jv3aw3HzY8VXJo6OdAlreZnGv0E0\/edit#\" title=\"\">Preparation of linear polyethyleneimine (PEI)<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1FaJqCEToKQfNh2Js7i3q9QosT1QUSRXwLUg8iN3rcxs\/edit?usp=sharing\" title=\"\">Transfection with PEI<\/a><\/li>\n<li>Production of recombinant adeno-associated viruses<\/li>\n<li>Culture of SF9 cells<\/li>\n<li>Production and expansion of recombinant Baculovirus<\/li>\n<li>Determining titers for baculovirus<\/li>\n<\/ul>\n<p><strong><u>Specialized culture conditions and growth assessment<\/u><\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1PdihWSTz47xEkxb9VLzm2PbrlxWCvKKdEPn1l-xEAGM\/edit?usp=sharing\" title=\"\">Culture and differentiation of 3T3L1 cells<\/a><\/li>\n<li><a href=\" https:\/\/docs.google.com\/document\/d\/1ZvzBQOWMAMGbTt5Lt-iDsbS9eb0Q1WZ25D6ot04YtQw\/edit?usp=sharing\" title=\"\">soft agar assay<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1irRFjfLANKEgQmlS6zjtj0mYJonjh68lSkiHgSOxHKM\/edit?usp=sharing\" title=\"\">Delipidating serum<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1bPYNnt5xfnfy3sTyLt5OwOAFXSnU8YRMh83bCsF9ASA\/edit?usp=sharing\" title=\"\">Embryoid body protocol (<\/a><a href=\"https:\/\/docs.google.com\/document\/d\/1bPYNnt5xfnfy3sTyLt5OwOAFXSnU8YRMh83bCsF9ASA\/edit?usp=sharing\" title=\"\">french<\/a><a href=\"https:\/\/docs.google.com\/document\/d\/1bPYNnt5xfnfy3sTyLt5OwOAFXSnU8YRMh83bCsF9ASA\/edit?usp=sharing\" title=\"\">)<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1vbTSM_5shhvvRxl8txGbWJ_2dFa9J_nRhyLOOdAyxqU\/edit?usp=sharing\" title=\"\">Alamar Blue assay with Rezazurin<\/a><\/li>\n<li>growth curve with crystal violet<\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1vCE7fIlr-V3KOYIg_Z3Dd4bv7aiVzn3Cy-xZBbYNSOc\/edit?usp=sharing\" title=\"\">MTT-test<\/a><\/li>\n<\/ul>\n<p><strong>Metabolic assessment<\/strong><\/p>\n<ul>\n<li>Oxygen consumption and extracellular acidification (Seahorse)<\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1gi41fJl5XWjzI_8mYanxFl74C0RlW-uOI6ysnwjpGDs\/edit?usp=sharing\" title=\"\">H2-DCFDA assay to analyze H2O2 by flow cytometry<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1CqPMD0vIqm_zGXUXzZEaChMFQIg-9gVFdriWDbVEP9A\/edit?usp=sharing\" title=\"\">Glucose consumption with tritiated glucose<\/a><\/li>\n<\/ul>\n<p><strong><u>Imaging<\/u>:<\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/16wOZo7bGATI1KYeierDuZfx8fiuN9Q6HyWen90vzoso\/edit?usp=sharing\" title=\"\">Staining cells for immunofluorescence<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1lY0w2HhdYYdFcKQPINt-S6YHYHz10rShZNUHqEZ2gPo\/edit?usp=sharing\" title=\"\">Cleaning coverslips before immunofluorescence<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1DrythChw1hT0B_AnWcZLeSEh9pEMWca6syNOq8l-OQc\/edit?usp=sharing\" title=\"\">MOWIOL embedding medium<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1wPsIE-y1YXiGmBNmgqp5Myg0kgGRPSbjhMtSqSAhTeQ\/edit?usp=sharing\" title=\"\">HE staining<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1wU0ACpNOfRgilWViYlLEHbsBWOPCG0zrbcJmg5eM0FI\/edit?usp=sharing\" title=\"\">Pappenheim staining<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1fdlCLF7pWbmy1q--oMQOjOqQTyVtKF3LUQJc345uo0I\/edit?usp=sharing\" title=\"\">Oil Red O staining<\/a><\/li>\n<\/ul>\n<p><strong><u>Flow cytometry<\/u><\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1wgXx54T9xgZnnD9t700rYUpzV5o0VN5RD0HDfi2xFdI\/edit?usp=sharing\" title=\"\">Annexin V and PI staining to determine apoptosis<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1HusG-xIZhauwS8YeyW7xnDt__4WVXGmiiUUZKsnwc-U\/edit?usp=sharing\" title=\"\">Double thymidine block<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1_D37fPlNaIBEi8BH4WHxLg8GmBVUlIQ1HlVWF9hVRfw\/edit?usp=sharing\" title=\"\">Thymidine-Nocodazole block<\/a><\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1lJE7THsHJywJ5sxeCG5AIqwVbGS0HWks1H2mxRH230c\/edit?usp=sharing\" title=\"\">EdU assay to assess cells in S-phase<\/a><\/li>\n<li>Usage of the FACSverse flow cytometer<\/li>\n<li>Usage of the Fortessa flow cytometer<\/li>\n<li><a href=\"https:\/\/docs.google.com\/document\/d\/1ykPquBzu5uDp42XvW7olonDYh7c-fRkVokiqeVP-BiY\/edit?usp=sharing\" title=\"\">Usage of FACScalibur<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><!-- \/divi:html --><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-213","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/chaletloree.com\/index.php\/wp-json\/wp\/v2\/pages\/213","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/chaletloree.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/chaletloree.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/chaletloree.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/chaletloree.com\/index.php\/wp-json\/wp\/v2\/comments?post=213"}],"version-history":[{"count":3,"href":"http:\/\/chaletloree.com\/index.php\/wp-json\/wp\/v2\/pages\/213\/revisions"}],"predecessor-version":[{"id":304,"href":"http:\/\/chaletloree.com\/index.php\/wp-json\/wp\/v2\/pages\/213\/revisions\/304"}],"wp:attachment":[{"href":"http:\/\/chaletloree.com\/index.php\/wp-json\/wp\/v2\/media?parent=213"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}